SrasV1, Main, Exploration, bibRecord, 003081

Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.

Identifieur interne : 003081 ( Main/Exploration ); précédent : 003080; suivant : 003082

Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.

Auteurs : Yanning Lu [Singapour] ; Ding Xiang Liu ; James P. Tam

Source :

Biochemical and biophysical research communications [ 1090-2104 ] ; 2008.

RBID : pubmed:18279660

Descripteurs français

KwdFr :
- Animaux, Cellules Vero, Microdomaines membranaires (virologie), Pénétration virale, Virus du SRAS (physiologie).
MESH :
- physiologie : Virus du SRAS.
- virologie : Microdomaines membranaires.
- Animaux, Cellules Vero, Pénétration virale.

English descriptors

KwdEn :
- Animals, Chlorocebus aethiops, Membrane Microdomains (virology), SARS Virus (physiology), Vero Cells, Virus Internalization.
MESH :
- physiology : SARS Virus.
- virology : Membrane Microdomains.
- Animals, Chlorocebus aethiops, Vero Cells, Virus Internalization.

Abstract

Lipid rafts often serve as an entry site for certain viruses. Here, we report that lipid rafts in Vero E6 cells are involved in the entry of severe acute respiratory syndrome coronavirus (SARS-CoV). Infectivity assay showed the integrity of lipid rafts was required for productive infection of pseudotyped SARS-CoV. Depletion of plasma membrane cholesterol with MbetaCD relocalized raft-resident marker caveolin-1 as well as SARS-CoV receptor ACE2 to a nonraft environment, but did not significantly change the surface expression of ACE2. MbetaCD-treatment inhibited infectivity of pseudotyped SARS-CoV by 90%. Biochemical fractionation and confocal imaging confirmed that ACE2 colocalized with raft-resident markers. Furthermore, an ectodomain of SARS-CoV S protein (S1188HA) could associate with lipid rafts after binding to its receptor, and colocalize with raft-resident marker ganglioside GM1. The binding of S1188HA was not affected by depleting plasma membrane cholesterol. Taken together, our results support that lipid rafts serve as an entry port for SARS-CoV.

DOI: 10.1016/j.bbrc.2008.02.023
PubMed: 18279660

Affiliations:

Singapour

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.</title>
<author><name sortKey="Lu, Yanning" sort="Lu, Yanning" uniqKey="Lu Y" first="Yanning" last="Lu">Yanning Lu</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name>
</author>
<author><name sortKey="Tam, James P" sort="Tam, James P" uniqKey="Tam J" first="James P" last="Tam">James P. Tam</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2008">2008</date>
<idno type="RBID">pubmed:18279660</idno>
<idno type="pmid">18279660</idno>
<idno type="doi">10.1016/j.bbrc.2008.02.023</idno>
<idno type="wicri:Area/PubMed/Corpus">001C11</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C11</idno>
<idno type="wicri:Area/PubMed/Curation">001C11</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001C11</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001A95</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001A95</idno>
<idno type="wicri:Area/Ncbi/Merge">001B80</idno>
<idno type="wicri:Area/Ncbi/Curation">001B80</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001B80</idno>
<idno type="wicri:Area/Main/Merge">003160</idno>
<idno type="wicri:Area/Main/Curation">003081</idno>
<idno type="wicri:Area/Main/Exploration">003081</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.</title>
<author><name sortKey="Lu, Yanning" sort="Lu, Yanning" uniqKey="Lu Y" first="Yanning" last="Lu">Yanning Lu</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name>
</author>
<author><name sortKey="Tam, James P" sort="Tam, James P" uniqKey="Tam J" first="James P" last="Tam">James P. Tam</name>
</author>
</analytic>
<series><title level="j">Biochemical and biophysical research communications</title>
<idno type="eISSN">1090-2104</idno>
<imprint><date when="2008" type="published">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Membrane Microdomains (virology)</term>
<term>SARS Virus (physiology)</term>
<term>Vero Cells</term>
<term>Virus Internalization</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Microdomaines membranaires (virologie)</term>
<term>Pénétration virale</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Microdomaines membranaires</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Membrane Microdomains</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Vero Cells</term>
<term>Virus Internalization</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Pénétration virale</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Lipid rafts often serve as an entry site for certain viruses. Here, we report that lipid rafts in Vero E6 cells are involved in the entry of severe acute respiratory syndrome coronavirus (SARS-CoV). Infectivity assay showed the integrity of lipid rafts was required for productive infection of pseudotyped SARS-CoV. Depletion of plasma membrane cholesterol with MbetaCD relocalized raft-resident marker caveolin-1 as well as SARS-CoV receptor ACE2 to a nonraft environment, but did not significantly change the surface expression of ACE2. MbetaCD-treatment inhibited infectivity of pseudotyped SARS-CoV by 90%. Biochemical fractionation and confocal imaging confirmed that ACE2 colocalized with raft-resident markers. Furthermore, an ectodomain of SARS-CoV S protein (S1188HA) could associate with lipid rafts after binding to its receptor, and colocalize with raft-resident marker ganglioside GM1. The binding of S1188HA was not affected by depleting plasma membrane cholesterol. Taken together, our results support that lipid rafts serve as an entry port for SARS-CoV.</div>
</front>
</TEI>
<affiliations><list><country><li>Singapour</li>
</country>
</list>
<tree><noCountry><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name>
<name sortKey="Tam, James P" sort="Tam, James P" uniqKey="Tam J" first="James P" last="Tam">James P. Tam</name>
</noCountry>
<country name="Singapour"><noRegion><name sortKey="Lu, Yanning" sort="Lu, Yanning" uniqKey="Lu Y" first="Yanning" last="Lu">Yanning Lu</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003081 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003081 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:18279660
   |texte=   Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:18279660" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.

Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.